Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hashimoto, H.; Hong, S.; Bhagwat, A.S.; Zhang, X.; Cheng, X.
    Excision of 5-hydroxymethyluracil and 5-carboxylcytosine by the thymine DNA glycosylase domain: its structural basis and implications for active DNA demethylation (2012), Nucleic Acids Res., 40, 10203-10214.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.29 expressed in Escherichia coli BL21(DE3)-Gold cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.29 in complex with a 28-base pair DNA containing a guanine:5-hydroxymethyluracil mismatch, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.29 5-carboxylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-formylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-hydroxymethyluracil-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O Homo sapiens
-
 thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O Homo sapiens
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.29 Homo sapiens Q13569
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.29 nickel-charged chelating column chromatography, HiTrap SP column chromatography, and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.29 5-carboxylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-formylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O
-
 Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.29 monomer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.2.29 TDG
-
 Homo sapiens
3.2.2.29 thymine DNA glycosylase
-
 Homo sapiens